UltraTag RBC

None known.

The kit should be stored at controlled room temperature 20° to 25°C (68° to 77°F). Syringe I should be protected from light if not stored in the kit tray.

Instructions for the Preparation of Technetium Tc 99m-Labeled Red Blood Cells Using Ultratag™ RBC

1. Collect patient's blood sample (1.0 to 3.0 mL) using heparin or ACD as an anticoagulant. The amount of ACD should not exceed 0.15 mL of ACD per mL of blood. The recommended amount of heparin is 10-15 units per mL of blood. DO NOT USE EDTA OR OXALATE AS AN ANTICOAGULANT.
2. Using a large-bore (19 to 21 gauge) needle, transfer 1.0 to 3.0 mL of anticoagulated whole blood to the reaction vial and gently mix to dissolve the lyophilized material. Allow to react for five minutes.
3. Add contents of Syringe I, mix by gently inverting four to five times.
4. Add the contents of Syringe II to the reaction vial. Mix by gently inverting four to five times.
5. Place the vial in a lead shield fitted with a lead cap and having a minimum wall thickness of 1/8 inch. Add 370 to 3700 MBq (10 to 100 mCi) sodium pertechnetate Tc 99m (in a volume of up to 3 mL) to the reaction vial. The avoidance of long technetium Tc 99 in-growth times and the use of fresh sodium pertechnetate Tc 99m generator eluate is recommended.
6. Mix by gently inverting reaction vial four to five times. Allow to react for 20 minutes with occasional mixing.
7. Technetium Tc 99m-labeled red blood cells should be injected within 30 minutes of preparation or as soon as possible thereafter.
8. If desired, assay labeling efficiency immediately prior to injection. Typical labeling efficiency is greater than 95%.
9. Mix gently prior to withdrawal of patient dose. Aseptically transfer the technetium Tc 99m-labeled red blood cells to a syringe for administration to the patient. Use largest bore needle compatible with patient administration to prevent hemolysis.
10. Assay the Tc 99m-labeled red blood cell patient dose in a suitable calibrator and complete the radioassay information label. Affix the radioassay information label to the shield.